A150V
Category 4 — Stable Fold, Function DisruptedConflictingCytoplasmic · predictedEditorialAlanine → Valine at position 150 in N-terminal cytoplasmic domain. ClinVar Conflicting including T2D. AlphaMissense 0.13 (below threshold) — AM under-call. DynaMut2 ΔΔG -0.94 (substantial).
Interactive 3D Structure
Bond changes · DynaMut2 interaction analysis
| Interaction type | Wild-type partner | Mutant partner | Status |
|---|---|---|---|
| Hydrogen bond | R146 | R146 | Preserved |
| Hydrogen bond | R147 | R147 | Preserved |
| Hydrogen bond | — | R152 | Gained |
| Polar contact | R146 | R146 | Preserved |
| Polar contact | R147 | R147 | Preserved |
| Polar contact | R152 | R152 | Preserved |
| Van der Waals | R152 | — | Lost |
Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.
Computational Predictions
This variant is in a water-facing region, which is the regime the stability predictor was built and benchmarked for. Ordinary predictor error still applies.
Clinical Evidence
Observed in the general population.
ClinVar classifies this variant as Conflicting classifications of pathogenicity for WFS1-related diabetes (autosomal dominant). These are dominant WFS1-related disorders and are clinically distinct from classic recessive Wolfram syndrome. This classification is not a statement about Wolfram syndrome severity.
- WFS1-related diabetesautosomal dominantsubmitted as: Type 2 diabetes mellitus
- Submitters conflict on this classification (1★). Treat the conditions above as submitted, not as documented phenotypes.
- Phenotype scope is interpretation of submitted records. The computed values on this card (AlphaMissense, DynaMut2 ΔΔG, pLDDT) are measurements of the model, not of a patient.
- Presentation cannot be generalised from neighbouring variants: one nucleotide over can present very differently. Structural similarity supports a mechanistic hypothesis, never a phenotype claim.
Review status: 1★ conflicting classifications. ClinVar "conditions" is the union of all submissions; 0–1★ entries are submitted conditions, not documented phenotypes.
Highest in East Asian: AF 0.268% (115 of 42,898 alleles), 20.3x the global figure. The global AF describes the general population, not the at-risk group.
No homozygotes reported in gnomAD v4. For a recessive allele this is expected and is not, on its own, evidence either way.
| Ancestry group | Allele freq. | AC / AN | Hom. | Carrier est. |
|---|---|---|---|---|
| East Asian | 0.268% | 115 / 42,898 | 0 | ~1 in 190 |
| South Asian | 0.053% | 46 / 85,994 | 0 | ~1 in 930 |
| Remaining individuals | 0.034% | 21 / 61,202 | 0 | ~1 in 1460 |
| African / African American | 0.0040% | 3 / 74,264 | 0 | ~1 in 12380 |
| European (non-Finnish) | 0.0020% | 23 / 1,162,188 | 0 | ~1 in 25260 |
Source: gnomAD r4, retrieved 2026-08-21. Values are allele frequencies. The carrier estimate is derived from the allele frequency (Hardy-Weinberg, ~2·AF) and is an approximation, not a published figure — where a published carrier frequency exists it outranks this column and is cited on the card.
Structural Context
Position 150 in N-term. Neighbors: ASP151 (2.4 Å), LEU149 (2.5 Å), ARG147 (3.8 Å — same R147 as R146C cluster region).
A150V volume mismatch near R146-R147 cluster. |ΔΔG| 0.94; AM 0.13 under-call; T2D confirms.
Druggability Assessment
Mechanism: volume mismatch near R146/R147 cluster. Therapeutic: same 146-150 cytoplasmic microregion.
Why this matters
Feed this card to Wolfram Intelligence
Download the A150V PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.