A180P

Category 3/4 — Most DruggableUncertain significanceCytoplasmic · predictedSource card

Alanine → Proline at position 180 · N-terminal cytoplasmic (intrinsically disordered) · WFS1 (Wolframin)

Interactive 3D Structure

Wild-type reference

Wild-type A180 — hydrogen bond to Y184

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DynaMut2 mutant · A180P

Mutant P180 — hydrogen bond to R177 lost (8 contacts lost)

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Bond changes · DynaMut2 interaction analysis

8 lost3 gained7 preserved

Interaction type	Wild-type partner	Mutant partner	Status
Hydrogen bond	V176	V176	Preserved
Hydrogen bond	R177	—	Lost
Hydrogen bond	M183	—	Lost
Hydrogen bond	Y184	Y184	Preserved
Hydrogen bond	F247	—	Lost
Polar contact	V176	V176	Preserved
Polar contact	R177	—	Lost
Polar contact	M183	—	Lost
Polar contact	Y184	Y184	Preserved
Polar contact	F247	F247	Preserved
Van der Waals	V176	—	Lost
Van der Waals	—	V182	Gained
Van der Waals	Y184	—	Lost
Hydrophobic	—	M183	Gained
Hydrophobic	F247	F247	Preserved
Hydrophobic	V248	—	Lost
Hydrophobic	T251	T251	Preserved
Hydrophobic	—	L381	Gained

Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.

Computational Predictions

DynaMut2 ΔΔG

0.66kcal/mol

Stabilising — mild

AlphaMissense

0.998

likely pathogenic

AlphaFold pLDDT

model confidence

Schema

Cat 3/4

Category 3/4 — Most Druggable

Clinical Evidence

ClinVar classificationUncertain significance

Review statuscriteria provided, single submitter

Associated conditions—

Population frequency (gnomAD v4)Absent from gnomAD v4

cDNA changec.538G>C

ClinVar accessionVCV004718918

Last evaluated2025/09/03 00:00

Not observed in ~730k individuals — consistent with a rare allele (ACMG PM2_supporting).

Full Variant Card

WFS1 Wolframin — A180P Variant Card

Molecular Atlas Pilot · RareResearch.AI · Generated by wolfram-variant-card skill

Alanine → Proline at position 180. N-terminal cytoplasmic (intrinsically disordered). ClinVar Uncertain significance, AlphaMissense 0.998, DynaMut2 ΔΔG +0.66 kcal/mol (stabilising).

Identity

Field	Value
Variant	A180P (p.Alanine180Proline)
DNA change	c.538G>C
Gene · Protein	WFS1 · Wolframin (890 aa)
UniProt	O76024 · WFS1_HUMAN
ClinVar accession	VCV004718918
Amino acid change	Alanine (A) → Proline (P)

Structural Context

Field	Value
AlphaFold model	AF-O76024-F1, v6
pLDDT at residue 180	90.00 — well-folded
Domain	N-terminal cytoplasmic (intrinsically disordered)
Position context	N-terminal cytoplasmic (intrinsically disordered)
IDR flag	No — pLDDT above 50 threshold

UniProt features at this position:

(none catalogued)

Position 180 sits in N-terminal cytoplasmic (intrinsically disordered). The wild-type residue is small/hydrophobic (alanine — methyl sidechain); the mutant is rigid/helix-breaking (proline — kinks backbone). The chemistry shift implies altered local packing, hydrogen-bonding, and/or electrostatics at this site.

Computational Predictions

AlphaMissense

Field	Value
am_pathogenicity	0.9982
am_class	likely pathogenic
Interpretation	Likely pathogenic (threshold 0.564)

DynaMut2

Field	Value
ΔΔG (kcal/mol)	0.66 (Stabilising)
Job ID	178090202722
Result URL	https://biosig.lab.uq.edu.au/dynamut2/results_prediction/178090202722

Clinical Evidence

Field	Value
Classification	Uncertain significance
Review status	criteria provided, single submitter
Last evaluated	2025/09/03 00:00
Inheritance	Inheritance pattern not specified in ClinVar entry; WFS1 has both AD and AR presentations.
WFS1 variant landscape	A180P is 1 of ~326 pathogenic-spectrum variants in WFS1 (out of 2,243 catalogued in ClinVar)

(no conditions catalogued)

Research Path Decision Tree

ΔΔG < 2  + binding site affected   →  CATEGORY 3 — docking experiments
ΔΔG 2–4                            →  CATEGORY 2 — pharmacological chaperones
ΔΔG > 4                            →  CATEGORY 1 — gene therapy
pLDDT < 50                         →  CATEGORY 5 — IDR, experimental only
Stable fold + functional site hit  →  CATEGORY 4 — site-specific docking

Final Schema Categorization

Category 3/4 — Most Druggable

<strong>Category 3/4 — Most Druggable</strong><br/><br/>|ΔΔG|=0.66 < 2 kcal/mol (fold intact) + AlphaMissense 0.998 confirms functional impact. Specific local contacts disrupted — priority for docking and pharmacological chaperone screening.

Why this card matters. Wolframin's fold survives this substitution (|ΔΔG|=0.66 kcal/mol). The pathogenic signal is real — AlphaMissense places it at 0.998. Protein still folds, but a specific local site is broken. Pharmacological chaperones and small-molecule binders are the rational therapeutic vector.

Files in this folder

AF-O76024-F1-model_v6.pdb — AlphaFold structure
A180P_molstar_viewer.html — interactive 3D viewer (auto-highlights position 180 with ball-and-stick + neighbors within 5Å)
A180P_variant_card.md — this card (source of truth)
A180P_variant_card.html — styled printable card
A180P_dynamut2_summary.html — clean offline DynaMut2 result card
dynamut2_result.json — structured result data
dynamut2_result_page.html — local snapshot of the Biosig result page (asset URLs absolutized)
A180P_wildtype_interactions.pse / A180P_mutant_interactions.pse — PyMOL sessions

Generated by wolfram-variant-card skill · RareResearch.AI Molecular Atlas Every assumption documented. Every score sourced.

Therapeutic Strategy Handoff · prediction

Feed this card to Wolfram Intelligence

Download the A180P PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.

Download A180P PDF card ↓Strategies are AI-generated predictions, not validated therapeutics.