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A787T

Category 5 — IDR ExclusionConflictingLumenal · predictedσ-1 candidateEditorial
AlanineThreonine at position 787 · C-terminal lumenal domain (653-869) · WFS1 (Wolframin)

Ala→Thr p787 lumenal AM=0.06 ddg=+0.05 pLDDT=45. ClinVar Conflicting evidence. Atlas mechanism: see structural analysis.

Interactive 3D Structure

Wild-type reference
Wild-type A787 — hydrogen bond to G789
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DynaMut2 mutant · A787T
Mutant T787 — hydrogen bond to G789 lost (3 contacts lost)
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Bond changes · DynaMut2 interaction analysis

3 lost0 gained0 preserved
Interaction typeWild-type partnerMutant partnerStatus
Hydrogen bondG789Lost
Polar contactS785Lost
Polar contactG789Lost

Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.

Computational Predictions

DynaMut2 ΔΔG
0.05kcal/mol
Stabilising — mild
AlphaMissense
0.063
LBen
AlphaFold pLDDT
45
model confidence
Schema
Cat 5
Category 5 — IDR Exclusion
ΔΔG confidence: Reduced confidence · Lumenal
  • pLDDT 44.69 below 70

The underlying AlphaFold model has pLDDT below 70 at this position, so the structure the stability calculation was run on is itself uncertain.

Do not rank this value against a variant from a different region on ΔΔG alone.

Clinical Evidence

ClinVar classificationConflicting classifications of pathogenicity
Review statuscriteria provided, conflicting classifications
Associated conditions(no specific conditions catalogued)
InheritanceConflicting ClinVar classifications.
Population frequency (gnomAD v4)Ultra-rare · AF 0.0079%
cDNA changec.2359G>A
ClinVar accessionVCV000444631
Last evaluated2026/01/09 00:00

Observed at very low frequency in gnomAD.

Classified for1★ unverified submission

ClinVar classifies this variant as Conflicting classifications of pathogenicity, but does not state what it is classified for. ClinVar records the condition only as "not specified", "not provided". A classification without a phenotype and an inheritance mode cannot be read as a statement about Wolfram syndrome risk or severity.

  • Phenotype scope is interpretation of submitted records. The computed values on this card (AlphaMissense, DynaMut2 ΔΔG, pLDDT) are measurements of the model, not of a patient.
  • Presentation cannot be generalised from neighbouring variants: one nucleotide over can present very differently. Structural similarity supports a mechanistic hypothesis, never a phenotype claim.

Review status: 1★ conflicting classifications. ClinVar "conditions" is the union of all submissions; 0–1★ entries are submitted conditions, not documented phenotypes.

Population frequency
Global (all ancestries)
AF 0.0079% · 127 / 1,612,958 alleles
Homozygotes
0
Highest-frequency population
Middle Eastern · AF 0.033%

Highest in Middle Eastern: AF 0.033% (2 of 6,062 alleles), 4.2x the global figure. The global AF describes the general population, not the at-risk group.

No homozygotes reported in gnomAD v4. For a recessive allele this is expected and is not, on its own, evidence either way.

Ancestry groupAllele freq.AC / ANHom.Carrier est.
Middle Eastern0.033%2 / 6,0620~1 in 1520
East Asian0.029%13 / 44,8720~1 in 1730
African / African American0.011%8 / 75,0740~1 in 4690
Finnish0.0080%5 / 62,8360~1 in 6280
European (non-Finnish)0.0074%87 / 1,179,9980~1 in 6780
South Asian0.0066%6 / 91,0880~1 in 7590
Remaining individuals0.0064%4 / 62,4880~1 in 7810
Admixed American0.0033%2 / 60,0280~1 in 15010

Source: gnomAD r4, retrieved 2026-08-21. Values are allele frequencies. The carrier estimate is derived from the allele frequency (Hardy-Weinberg, ~2·AF) and is an approximation, not a published figure — where a published carrier frequency exists it outranks this column and is cited on the card.

Structural Context

Position analysis: ASP788 (2.5 Å — partner of G789S!), GLY786 (2.6 Å), SER785 (4.4 Å). pLDDT 45 IDR boundary. Same region as S790L/W cluster. The Atlas's neighbor extraction surfaces this variant's contacts and connects them to the broader multi-variant target landscape.

Amino-acid chemistry
polarity introduced
Position in the protein
C-terminal lumenal IDR boundary

Druggability Assessment

Cat 5 IDR boundary — see structural prose. AlphaMissense below threshold (AM under-call class) but mechanism is structurally identified. Therapeutic strategy: site-directed at contacts identified above, or wet-lab validation if pLDDT borderline/below 50.

Why this matters

IDR boundary near S790 cluster.
Therapeutic Strategy Handoff · prediction

Feed this card to Wolfram Intelligence

Download the A787T PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.

Download A787T PDF card ↓Strategies are AI-generated predictions, not validated therapeutics.

UniProt Domain Annotation

Chain1890 · Wolframin
Topological domain653869 · Lumenal