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D797V

Category 3/4 — Most DruggableConflictingLumenal · predictedσ-1 candidateEditorial
AspartateValine at position 797 · C-terminal lumenal domain (653-869) · WFS1 (Wolframin)

Aspartate → Valine at position 797 in lumenal domain. ClinVar Conflicting. AlphaMissense 0.876, ΔΔG +0.04 (neutral). pLDDT 65 borderline.

Interactive 3D Structure

Wild-type reference
Wild-type D797 — hydrogen bond to K800
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DynaMut2 mutant · D797V
Mutant V797 — hydrogen bond to T799 lost (2 contacts lost)
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Bond changes · DynaMut2 interaction analysis

2 lost2 gained5 preserved
Interaction typeWild-type partnerMutant partnerStatus
Hydrogen bondE794E794Preserved
Hydrogen bondT799Lost
Hydrogen bondK800K800Preserved
Polar contactE794E794Preserved
Polar contactT799T799Preserved
Polar contactK800K800Preserved
Van der WaalsT799Gained
HydrophobicT799Gained
HydrophobicK800Lost

Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.

Computational Predictions

DynaMut2 ΔΔG
0.04kcal/mol
Stabilising — mild
AlphaMissense
0.876
LPath
AlphaFold pLDDT
65
model confidence
Schema
Cat 3/4
Category 3/4 — Most Druggable

Clinical Evidence

ClinVar classificationConflicting classifications of pathogenicity
Review statuscriteria provided, conflicting classifications
Associated conditions(no specific conditions catalogued)
InheritanceNot specified.
Population frequency (gnomAD v4)Absent from gnomAD v4
cDNA changec.2390A>T
ClinVar accessionVCV002203531
Last evaluated2025/03/31 00:00

Not observed in ~730k individuals — consistent with a rare allele (ACMG PM2_supporting).

Structural Context

Position 797 sits in the lumenal C-terminal region. Neighbors: VAL798 (2.4 Å), ASP796 (2.4 Å — adjacent aspartate), GLU794 (4.2 Å), THR799 (4.3 Å).

Replacing D797 with valine eliminates the negative charge in a local charged cluster (D796, E794 nearby). Fold accommodates (ΔΔG essentially zero). AlphaMissense 0.876 confirms severe consequence. Same position as D797N — both pathogenic.

Amino-acid chemistry
Aspartate (D) → Valine (V) — negatively-charged carboxylate replaced by branched aliphatic hydrophobic.
Position in the protein
C-terminal lumenal domain · position 797 (pLDDT 65 borderline).

Druggability Assessment

Category 4 — Stable Fold, Function Disrupted (pLDDT caveat). ΔΔG ≈ 0. AlphaMissense 0.876 confirms severe consequence. pLDDT 65 borderline.

Mechanism: charge loss from local D796-D797-E794 cluster. Therapeutic: site-directed at this charge cluster.

Why this matters

D797V + D797N at same position. Charge cluster D796-D797-E794 is a recognition surface; multiple variants disrupt it.
Therapeutic Strategy Handoff · prediction

Feed this card to Wolfram Intelligence

Download the D797V PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.

Download D797V PDF card ↓Strategies are AI-generated predictions, not validated therapeutics.

UniProt Domain Annotation

Chain1890 · Wolframin
Topological domain653869 · Lumenal
Natural variant797797 · in WFSL