# WFS1 Wolframin — E694D Variant Card

**Molecular Atlas Pilot · RareResearch.AI · Generated by wolfram-variant-card skill**

*Glutamic acid → Aspartic acid at position 694. C-terminal ER-lumenal (calcium binding. ClinVar Uncertain significance/Uncertain risk allele, AlphaMissense 0.668, DynaMut2 ΔΔG -0.21 kcal/mol (destabilising).*

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## Identity

| Field | Value |
|---|---|
| **Variant** | E694D (p.Glutamic acid694Aspartic acid) |
| **DNA change** | c.2082G>C |
| **Gene · Protein** | WFS1 · Wolframin (890 aa) |
| **UniProt** | O76024 · WFS1_HUMAN |
| **ClinVar accession** | VCV000918068 |
| **Amino acid change** | Glutamic acid (E) → Aspartic acid (D) |

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## Structural Context

| Field | Value |
|---|---|
| **AlphaFold model** | AF-O76024-F1, v6 |
| **pLDDT at residue 694** | **86.12** — well-folded |
| **Domain** | C-terminal ER-lumenal (calcium binding, calmodulin, chaperone) |
| **Position context** | C-terminal lumenal domain · position 694 projects into the ER lumen |
| **IDR flag** | No — pLDDT above 50 threshold |

**UniProt features at this position:**

  (none catalogued)

> Position 694 sits in the C-terminal lumenal domain (residues 653–869), wolframin's largest soluble region. This domain projects into the ER lumen and is implicated in calcium handling, ER stress sensing, and protein–protein interactions with ATF6 and Na+/K+ ATPase β1. The wild-type residue is negatively charged (glutamate — carboxylate); the mutant is negatively charged (aspartate — carboxylate). The chemistry shift implies altered local packing, hydrogen-bonding, and/or electrostatics at this site.

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## Computational Predictions

### AlphaMissense
| Field | Value |
|---|---|
| am_pathogenicity | **0.6683** |
| am_class | **likely pathogenic** |
| Interpretation | Likely pathogenic (threshold 0.564) |

### DynaMut2
| Field | Value |
|---|---|
| ΔΔG (kcal/mol) | **-0.21 (Destabilising)** |
| Job ID | 178092127466 |
| Result URL | https://biosig.lab.uq.edu.au/dynamut2/results_prediction/178092127466 |

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## Clinical Evidence

| Field | Value |
|---|---|
| Classification | **Uncertain significance/Uncertain risk allele** |
| Review status | criteria provided, multiple submitters, no conflicts |
| Last evaluated | 2024/05/10 00:00 |
| Inheritance | Autosomal dominant pattern indicated by associated DFNA6/14/38 (WFS1 hearing loss 6). |
| WFS1 variant landscape | E694D is 1 of ~326 pathogenic-spectrum variants in WFS1 (out of 2,243 catalogued in ClinVar) |

- Cataract 41
- Autosomal dominant nonsyndromic hearing loss 6
- Type 2 diabetes mellitus
- Wolfram syndrome 1
- Wolfram-like syndrome

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## Research Path Decision Tree

```
ΔΔG < 2  + binding site affected   →  CATEGORY 3 — docking experiments
ΔΔG 2–4                            →  CATEGORY 2 — pharmacological chaperones
ΔΔG > 4                            →  CATEGORY 1 — gene therapy
pLDDT < 50                         →  CATEGORY 5 — IDR, experimental only
Stable fold + functional site hit  →  CATEGORY 4 — site-specific docking
```

## Final Schema Categorization

**Category 3/4 — Most Druggable**

<strong>Category 3/4 — Most Druggable</strong><br/><br/>|ΔΔG|=0.21 < 2 kcal/mol (fold intact) + AlphaMissense 0.668 confirms functional impact. Specific local contacts disrupted — priority for docking and pharmacological chaperone screening.

**Why this card matters.** Wolframin's fold survives this substitution (|ΔΔG|=0.21 kcal/mol). The pathogenic signal is real — AlphaMissense places it at 0.668. Protein still folds, but a specific local site is broken. Pharmacological chaperones and small-molecule binders are the rational therapeutic vector.

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## Files in this folder

- `AF-O76024-F1-model_v6.pdb` — AlphaFold structure
- `E694D_molstar_viewer.html` — interactive 3D viewer (auto-highlights position 694 with ball-and-stick + neighbors within 5Å)
- `E694D_variant_card.md` — this card (source of truth)
- `E694D_variant_card.html` — styled printable card
- `E694D_dynamut2_summary.html` — clean offline DynaMut2 result card
- `dynamut2_result.json` — structured result data
- `dynamut2_result_page.html` — local snapshot of the Biosig result page (asset URLs absolutized)
- `E694D_wildtype_interactions.pse` / `E694D_mutant_interactions.pse` — PyMOL sessions

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*Generated by wolfram-variant-card skill · RareResearch.AI Molecular Atlas*
*Every assumption documented. Every score sourced.*