# WFS1 Wolframin — K252E Variant Card

**Molecular Atlas Pilot · RareResearch.AI · Generated by wolfram-variant-card skill**

*Lysine → Glutamate at position 252 in N-terminal cytoplasmic domain. ClinVar Conflicting including Wolfram syndrome 1. AlphaMissense 0.872, ΔΔG -1.26. Charge-flip variant in cytoplasmic domain.*

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## Identity

| Field | Value |
|---|---|
| **Variant** | K252E (p.Lysine252Glutamate) |
| **DNA change** | c.754A>G |
| **Gene · Protein** | WFS1 · Wolframin (890 aa) |
| **UniProt** | O76024 · WFS1_HUMAN |
| **ClinVar accession** | VCV000505263 |
| **Amino acid change** | Lysine (K) → Glutamate (E) — positively-charged amine replaced by negatively-charged carboxylate. Complete charge reversal. |

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## Structural Context

| Field | Value |
|---|---|
| **AlphaFold model** | AF-O76024-F1, v6 |
| **pLDDT at residue 252** | **87.31** — well-folded |
| **Domain** | N-terminal cytoplasmic domain (87-313) |
| **Position context** | N-terminal cytoplasmic domain · position 252 (pLDDT 87). |
| **IDR flag** | No — pLDDT above 50 threshold |

**UniProt features at this position:**

  - Chain: 1-890 Wolframin
  - Region: 1-321 Interaction with ATP6V1A

> Position 252 sits in cytoplasmic domain. Neighbors: LYS253 (2.5 Å — adjacent existing lysine!), THR251 (2.5 Å), GLU249 (3.7 Å — likely salt-bridge partner with wild-type K252), VAL248 (3.7 Å).

Replacing K252 with glutamate creates two negative charges (new E252 + existing E249) where wild-type had a positive K252 + negative E249 salt bridge. The local electrostatic surface flips polarity. |ΔΔG| 1.26 reflects substantial cost. AlphaMissense 0.872 + Wolfram 1 confirm severe consequence.

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## Computational Predictions

### AlphaMissense
| Field | Value |
|---|---|
| am_pathogenicity | **0.8722** |
| am_class | **LPath** |
| Interpretation | Likely pathogenic (threshold 0.564) |

### DynaMut2
| Field | Value |
|---|---|
| ΔΔG (kcal/mol) | **-1.26 (Destabilising)** |
| Job ID | 177992463158 |
| Result URL | https://biosig.lab.uq.edu.au/dynamut2/results_prediction/177992463158 |

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## Clinical Evidence

| Field | Value |
|---|---|
| Classification | **Conflicting classifications of pathogenicity** |
| Review status | criteria provided, conflicting classifications |
| Last evaluated | 2016/09/11 00:00 |
| Inheritance | Wolfram syndrome 1. |
| WFS1 variant landscape | K252E is 1 of ~326 pathogenic-spectrum variants in WFS1 (out of 2,243 catalogued in ClinVar) |

- Wolfram syndrome 1

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## Research Path Decision Tree

```
ΔΔG < 2  + binding site affected   →  CATEGORY 3 — docking experiments
ΔΔG 2–4                            →  CATEGORY 2 — pharmacological chaperones
ΔΔG > 4                            →  CATEGORY 1 — gene therapy
pLDDT < 50                         →  CATEGORY 5 — IDR, experimental only
Stable fold + functional site hit  →  CATEGORY 4 — site-specific docking
```

## Final Schema Categorization

**Category 3/4 — Most Druggable**

<strong>Category 3/4 — Most Druggable.</strong> |ΔΔG| = 1.26 — fold survives at meaningful cost. AlphaMissense 0.872 + Wolfram 1 confirm severe consequence.<br/><br/>Mechanism: charge-flip disrupting K252-E249 salt bridge + creating two-glutamate cluster. Therapeutic: site-directed at the 249-253 microregion.

**Why this card matters.** K252E continues the charge-flip class (with E169K, E809K, E864K, K705E). Recognition-surface disruption through charge sign reversal.

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## Files in this folder

- `AF-O76024-F1-model_v6.pdb` — AlphaFold structure
- `K252E_molstar_viewer.html` — interactive 3D viewer (auto-highlights position 252 with ball-and-stick + neighbors within 5Å)
- `K252E_variant_card.md` — this card (source of truth)
- `K252E_variant_card.html` — styled printable card
- `K252E_dynamut2_summary.html` — clean offline DynaMut2 result card
- `dynamut2_result.json` — structured result data
- `dynamut2_result_page.html` — local snapshot of the Biosig result page (asset URLs absolutized)
- `K252E_wildtype_interactions.pse` / `K252E_mutant_interactions.pse` — PyMOL sessions

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*Generated by wolfram-variant-card skill · RareResearch.AI Molecular Atlas*
*Every assumption documented. Every score sourced.*