K287N
Category 3/4 — Most DruggableUnknownCytoplasmic · predictedSource cardInteractive 3D Structure
Bond changes · DynaMut2 interaction analysis
| Interaction type | Wild-type partner | Mutant partner | Status |
|---|---|---|---|
| Ionic bond | E298 | — | Lost |
| Hydrogen bond | P279 | — | Lost |
| Hydrogen bond | L282 | — | Lost |
| Hydrogen bond | P283 | P283 | Preserved |
| Hydrogen bond | L284 | L284 | Preserved |
| Hydrogen bond | Y291 | Y291 | Preserved |
| Hydrogen bond | E298 | — | Lost |
| Polar contact | P279 | — | Lost |
| Polar contact | P283 | P283 | Preserved |
| Polar contact | L284 | L284 | Preserved |
| Polar contact | V289 | — | Lost |
| Polar contact | Y291 | Y291 | Preserved |
| Polar contact | E298 | — | Lost |
| Carbonyl | Y291 | Y291 | Preserved |
Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.
Computational Predictions
Clinical Evidence
Not observed in ~730k individuals — consistent with a rare allele (ACMG PM2_supporting).
Full Variant Card
WFS1 Wolframin — K287N Variant Card
Molecular Atlas Pilot · RareResearch.AI · Generated by wolfram-variant-card skill
Lysine (K) → Asparagine (N) at position 287
Identity
| Field | Value |
|---|---|
| Variant | K287N (p.Lysine287Asparagine) |
| DNA change | c.861G>T |
| Gene · Protein | WFS1 · Wolframin (890 aa) |
| UniProt | O76024 · WFS1_HUMAN |
| ClinVar accession | VCV004306964 |
| Amino acid change | Lysine (K) → Asparagine (N) |
Structural Context
| Field | Value |
|---|---|
| AlphaFold model | AF-O76024-F1, v6 |
| pLDDT at residue 287 | 53.44 — confident |
| Domain | N-terminal cytoplasmic (intrinsically disordered) |
| Position context | N-terminal cytoplasmic (intrinsically disordered) |
| IDR flag | No — pLDDT above 50 threshold |
UniProt features at this position:
(none catalogued)
Computational Predictions
AlphaMissense
| Field | Value |
|---|---|
| am_pathogenicity | 0.9311 |
| am_class | likely pathogenic |
| Interpretation | Likely pathogenic (threshold 0.564) |
DynaMut2
| Field | Value |
|---|---|
| ΔΔG (kcal/mol) | -0.78 (Destabilising) |
| Job ID | 178092103937 |
| Result URL | https://biosig.lab.uq.edu.au/dynamut2/results_prediction/178092103937 |
Clinical Evidence
| Field | Value |
|---|---|
| Classification | None |
| Review status | None |
| Last evaluated | 1/01/01 00:00 |
| Inheritance | — |
| WFS1 variant landscape | K287N is 1 of ~326 pathogenic-spectrum variants in WFS1 (out of 2,243 catalogued in ClinVar) |
(no conditions catalogued)
Research Path Decision Tree
ΔΔG < 2 + binding site affected → CATEGORY 3 — docking experiments
ΔΔG 2–4 → CATEGORY 2 — pharmacological chaperones
ΔΔG > 4 → CATEGORY 1 — gene therapy
pLDDT < 50 → CATEGORY 5 — IDR, experimental only
Stable fold + functional site hit → CATEGORY 4 — site-specific docking
Final Schema Categorization
Category 3/4 — Most Druggable
Mildly destabilizing fold; AlphaMissense confirms functional impact. Fold intact, specific local contacts/sites disrupted. Priority for docking and pharmacological chaperone screening.
Files in this folder
AF-O76024-F1-model_v6.pdb— AlphaFold structureK287N_molstar_viewer.html— interactive 3D viewer (auto-highlights position 287 with ball-and-stick + neighbors within 5Å)K287N_variant_card.md— this card (source of truth)K287N_variant_card.html— styled printable cardK287N_dynamut2_summary.html— clean offline DynaMut2 result carddynamut2_result.json— structured result datadynamut2_result_page.html— local snapshot of the Biosig result page (asset URLs absolutized)K287N_wildtype_interactions.pse/K287N_mutant_interactions.pse— PyMOL sessions
Generated by wolfram-variant-card skill · RareResearch.AI Molecular Atlas Every assumption documented. Every score sourced.
Feed this card to Wolfram Intelligence
Download the K287N PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.