DynaMut2 Result · RareResearch.AI Molecular Atlas
WFS1 R558C — Predicted Stability Change
Computed on AlphaFold AF-O76024-F1 v6 · Job ID 177985627697 · Biosig Lab, University of Queensland / Melbourne
ΔΔG (Stability Change)
-0.5kcal/mol
Destabilising
Mutation Details
- Chain
- A
- Position
- 558
- Wild-type
- R (Arginine)
- Mutant
- C (Cysteine)
- Region
- Cytoplasmic loop between TM7 (529-549) and TM8 (563-583)
Mechanistic Interpretation
Wild-type R558's guanidinium group makes specific ionic and polar contacts with neighboring TM7/TM8 residues. The R→C swap removes the positive charge and introduces a free thiol, eliminating those contacts and reorganizing the local hydrogen-bond network. Fold survives; local function disrupted.