T455K

Category 3/4 — Most DruggableUncertain significanceTransmembrane · predictedSource card

Threonine → Lysine at position 455 · Cytoplasmic loop 3 · WFS1 (Wolframin)

Interactive 3D Structure

Wild-type reference

Wild-type T455 — hydrogen bond to E452

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DynaMut2 mutant · T455K

Mutant K455 — hydrogen bond to E452 lost (4 contacts lost)

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Bond changes · DynaMut2 interaction analysis

4 lost9 gained8 preserved

Interaction type	Wild-type partner	Mutant partner	Status
Hydrogen bond	A451	A451	Preserved
Hydrogen bond	E452	—	Lost
Hydrogen bond	—	A458	Gained
Hydrogen bond	L459	L459	Preserved
Hydrogen bond	—	C537	Gained
Polar contact	A451	A451	Preserved
Polar contact	E452	E452	Preserved
Polar contact	—	R457	Gained
Polar contact	A458	A458	Preserved
Polar contact	L459	L459	Preserved
Polar contact	—	C537	Gained
Van der Waals	E452	—	Lost
Van der Waals	—	R457	Gained
Van der Waals	—	L459	Gained
Van der Waals	—	Y534	Gained
Hydrophobic	—	Y444	Gained
Hydrophobic	L447	L447	Preserved
Hydrophobic	A451	—	Lost
Hydrophobic	—	L459	Gained
Hydrophobic	M518	—	Lost
Hydrophobic	Y534	Y534	Preserved

Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.

Computational Predictions

DynaMut2 ΔΔG

-0.52kcal/mol

Destabilising — mild

AlphaMissense

0.895

likely pathogenic

AlphaFold pLDDT

model confidence

Schema

Cat 3/4

Category 3/4 — Most Druggable

Clinical Evidence

ClinVar classificationUncertain significance

Review statuscriteria provided, single submitter

Associated conditions—

Population frequency (gnomAD v4)Ultra-rare · AF 0.00025%

cDNA changec.1364C>A

ClinVar accessionVCV002053650

Last evaluated2022/04/10 00:00

Observed at very low frequency in gnomAD.

Full Variant Card

WFS1 Wolframin — T455K Variant Card

Molecular Atlas Pilot · RareResearch.AI · Generated by wolfram-variant-card skill

Threonine → Lysine at position 455. Cytoplasmic loop 3. ClinVar Uncertain significance, AlphaMissense 0.895, DynaMut2 ΔΔG -0.52 kcal/mol (destabilising).

Identity

Field	Value
Variant	T455K (p.Threonine455Lysine)
DNA change	c.1364C>A
Gene · Protein	WFS1 · Wolframin (890 aa)
UniProt	O76024 · WFS1_HUMAN
ClinVar accession	VCV002053650
Amino acid change	Threonine (T) → Lysine (K)

Structural Context

Field	Value
AlphaFold model	AF-O76024-F1, v6
pLDDT at residue 455	83.62 — well-folded
Domain	Cytoplasmic loop 3
Position context	Loop region · position 455 sits between transmembrane segments, solvent-accessible
IDR flag	No — pLDDT above 50 threshold

UniProt features at this position:

(none catalogued)

Position 455 sits in a connecting loop between transmembrane helices. Loop residues are typically solvent-exposed and often contribute to interhelical contacts or serve as recognition sites for binding partners. The wild-type residue is small polar (threonine — hydroxyl); the mutant is positively charged (lysine — primary amine). The chemistry shift implies altered local packing, hydrogen-bonding, and/or electrostatics at this site.

Computational Predictions

AlphaMissense

Field	Value
am_pathogenicity	0.8952
am_class	likely pathogenic
Interpretation	Likely pathogenic (threshold 0.564)

DynaMut2

Field	Value
ΔΔG (kcal/mol)	-0.52 (Destabilising)
Job ID	178092108319
Result URL	https://biosig.lab.uq.edu.au/dynamut2/results_prediction/178092108319

Clinical Evidence

Field	Value
Classification	Uncertain significance
Review status	criteria provided, single submitter
Last evaluated	2022/04/10 00:00
Inheritance	Inheritance pattern not specified in ClinVar entry; WFS1 has both AD and AR presentations.
WFS1 variant landscape	T455K is 1 of ~326 pathogenic-spectrum variants in WFS1 (out of 2,243 catalogued in ClinVar)

(no conditions catalogued)

Research Path Decision Tree

ΔΔG < 2  + binding site affected   →  CATEGORY 3 — docking experiments
ΔΔG 2–4                            →  CATEGORY 2 — pharmacological chaperones
ΔΔG > 4                            →  CATEGORY 1 — gene therapy
pLDDT < 50                         →  CATEGORY 5 — IDR, experimental only
Stable fold + functional site hit  →  CATEGORY 4 — site-specific docking

Final Schema Categorization

Category 3/4 — Most Druggable

<strong>Category 3/4 — Most Druggable</strong><br/><br/>|ΔΔG|=0.52 < 2 kcal/mol (fold intact) + AlphaMissense 0.895 confirms functional impact. Specific local contacts disrupted — priority for docking and pharmacological chaperone screening.

Why this card matters. Wolframin's fold survives this substitution (|ΔΔG|=0.52 kcal/mol). The pathogenic signal is real — AlphaMissense places it at 0.895. Protein still folds, but a specific local site is broken. Pharmacological chaperones and small-molecule binders are the rational therapeutic vector.

Files in this folder

AF-O76024-F1-model_v6.pdb — AlphaFold structure
T455K_molstar_viewer.html — interactive 3D viewer (auto-highlights position 455 with ball-and-stick + neighbors within 5Å)
T455K_variant_card.md — this card (source of truth)
T455K_variant_card.html — styled printable card
T455K_dynamut2_summary.html — clean offline DynaMut2 result card
dynamut2_result.json — structured result data
dynamut2_result_page.html — local snapshot of the Biosig result page (asset URLs absolutized)
T455K_wildtype_interactions.pse / T455K_mutant_interactions.pse — PyMOL sessions

Generated by wolfram-variant-card skill · RareResearch.AI Molecular Atlas Every assumption documented. Every score sourced.

Therapeutic Strategy Handoff · prediction

Feed this card to Wolfram Intelligence

Download the T455K PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.

Download T455K PDF card ↓Strategies are AI-generated predictions, not validated therapeutics.