# A133= — WFS1 Molecular Atlas Card

**Variant type:** Synonymous (silent)
**Codon:** position 133 (Alanine, A) — amino acid unchanged
**Domain context:** N-terminal cytoplasmic (intrinsically disordered)

---

## Schema category: **Silent — Silent — no amino-acid change**

No amino-acid change (A133 is unchanged): the codon is altered but the protein sequence is identical to wild-type. No structural, stability or AlphaMissense effect applies. Synonymous variants are typically benign unless they affect splicing or regulatory elements; this one is not adjacent to an exon boundary.

---

## Clinical evidence

- **Classification:** Benign/Likely benign
- **Review status:** criteria provided, multiple submitters, no conflicts
- **Associated conditions:** Wolfram syndrome 1
- **cDNA change:** c.399C>T
- **ClinVar accession:** VCV000750433
- **Last evaluated:** 2024/11/27 00:00
- **Submissions:** 1

---

_Card generated by `wolfram-atlas-batch` (synonymous pipeline) on 2026-06-08T02:51:02.647138Z._
_WFS1: UniProt O76024, AlphaFold v6. Synonymous variants carry no protein-structural effect._