# c.832+1G_A — WFS1 Molecular Atlas Card

**Variant type:** Splice site
**Boundary:** donor (5' splice site) · intronic offset +1
**Nearest protein position:** ~278 (N-terminal cytoplasmic (intrinsically disordered))

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## Schema category: **S3 — SpliceAI could not score this variant — wet-lab validation**

SpliceAI returned no usable prediction (no genomic coordinate / SpliceAI record). The canonical splice-region call stands; wet-lab RNA validation is required to resolve the isoform.

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## Splice prediction

- **Affected site:** donor (5' splice site), canonical (±1/±2 core)
- **SpliceAI:** no genomic coordinate / SpliceAI record

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## Clinical evidence

_Not found in the cached WFS1 ClinVar set (`_reference/WFS1_clinvar_variants.csv`)._

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_Card generated by `wolfram-atlas-batch` (splice pipeline) on 2026-06-08T07:53:13.337833Z._
_Schema: `reference/card_schema_extension.md` (S1–S3). WFS1: UniProt O76024, AlphaFold v6._