# c.861+10C_T — WFS1 Molecular Atlas Card

**Variant type:** Splice site
**Boundary:** donor (5' splice site) · intronic offset +10
**Nearest protein position:** ~287 (N-terminal cytoplasmic (intrinsically disordered))

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## Schema category: **S3 — Minimal predicted splicing impact (SpliceAI ΔS 0.00)**

SpliceAI predicts little splicing disruption at this donor (5') site (max ΔS 0.00 < 0.2; acceptor-gain 0.00, acceptor-loss 0.00, donor-gain 0.00, donor-loss 0.00). The variant may be tolerated or act through a weak/again-tissue-specific mechanism; wet-lab RNA validation is the arbiter before any therapeutic call.

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## Splice prediction

- **Affected site:** donor (5' splice site), extended splice region
- **SpliceAI delta scores** (GRCh38 chr4:6295199 C>T):
  - acceptor gain **0.00** · acceptor loss **0.00**
  - donor gain **0.00** · donor loss **0.00**
- **Predicted outcome:** Minimal predicted splicing impact (SpliceAI ΔS 0.00)

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## Clinical evidence

- **Classification:** Likely benign
- **Review status:** criteria provided, single submitter
- **cDNA change:** c.861+10C>T
- **ClinVar accession:** VCV004694261
- **Last evaluated:** 2025/03/13 00:00
- **Submissions:** 1

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_Card generated by `wolfram-atlas-batch` (splice pipeline) on 2026-06-08T07:53:16.916584Z._
_Schema: `reference/card_schema_extension.md` (S1–S3). WFS1: UniProt O76024, AlphaFold v6._