L286P

Category 4 — Stable Fold, Function DisruptedUncertain significanceCytoplasmic · predictedSource card

Leucine → Proline at position 286 · N-terminal cytoplasmic (intrinsically disordered) · WFS1 (Wolframin)

Interactive 3D Structure

Wild-type reference

Wild-type L286 — hydrogen bond to K290

Fullscreen ↗

DynaMut2 mutant · L286P

Mutant P286 — hydrogen bond to P283 lost (2 contacts lost)

Fullscreen ↗

Bond changes · DynaMut2 interaction analysis

2 lost2 gained7 preserved

Interaction type	Wild-type partner	Mutant partner	Status
Hydrogen bond	P283	P283	Preserved
Hydrogen bond	V289	V289	Preserved
Hydrogen bond	K290	K290	Preserved
Polar contact	P283	P283	Preserved
Polar contact	L284	—	Lost
Polar contact	V289	V289	Preserved
Polar contact	K290	K290	Preserved
Van der Waals	—	P283	Gained
Van der Waals	L284	—	Lost
Van der Waals	K290	K290	Preserved
Hydrophobic	—	P283	Gained

Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.

Computational Predictions

DynaMut2 ΔΔG

-0.24kcal/mol

Destabilising — mild

AlphaMissense

0.344

ambiguous

AlphaFold pLDDT

model confidence

Schema

Cat 4

Category 4 — Stable Fold, Function Disrupted

Clinical Evidence

ClinVar classificationUncertain significance

Review statuscriteria provided, multiple submitters, no conflicts

Associated conditionsWFS1-related disorder; Inborn genetic diseases

Population frequency (gnomAD v4)Absent from gnomAD v4

cDNA changec.857T>C

ClinVar accessionVCV002634718

Last evaluated2024/11/10 00:00

Not observed in ~730k individuals — consistent with a rare allele (ACMG PM2_supporting).

Full Variant Card

WFS1 Wolframin — L286P Variant Card

Molecular Atlas Pilot · RareResearch.AI · Generated by wolfram-variant-card skill

Leucine → Proline at position 286. N-terminal cytoplasmic (intrinsically disordered). ClinVar Uncertain significance, AlphaMissense 0.344, DynaMut2 ΔΔG -0.24 kcal/mol (destabilising).

Identity

Field	Value
Variant	L286P (p.Leucine286Proline)
DNA change	c.857T>C
Gene · Protein	WFS1 · Wolframin (890 aa)
UniProt	O76024 · WFS1_HUMAN
ClinVar accession	VCV002634718
Amino acid change	Leucine (L) → Proline (P)

Structural Context

Field	Value
AlphaFold model	AF-O76024-F1, v6
pLDDT at residue 286	56.12 — confident
Domain	N-terminal cytoplasmic (intrinsically disordered)
Position context	N-terminal cytoplasmic (intrinsically disordered)
IDR flag	No — pLDDT above 50 threshold

UniProt features at this position:

(none catalogued)

Position 286 sits in N-terminal cytoplasmic (intrinsically disordered). The wild-type residue is medium hydrophobic (leucine — branched); the mutant is rigid/helix-breaking (proline — kinks backbone). The chemistry shift implies altered local packing, hydrogen-bonding, and/or electrostatics at this site.

Computational Predictions

AlphaMissense

Field	Value
am_pathogenicity	0.3439
am_class	ambiguous
Interpretation	Likely benign (threshold 0.564)

DynaMut2

Field	Value
ΔΔG (kcal/mol)	-0.24 (Destabilising)
Job ID	178094725006
Result URL	https://biosig.lab.uq.edu.au/dynamut2/results_prediction/178094725006

Clinical Evidence

Field	Value
Classification	Uncertain significance
Review status	criteria provided, multiple submitters, no conflicts
Last evaluated	2024/11/10 00:00
Inheritance	Inheritance pattern not specified in ClinVar entry; WFS1 has both AD and AR presentations.
WFS1 variant landscape	L286P is 1 of ~326 pathogenic-spectrum variants in WFS1 (out of 2,243 catalogued in ClinVar)

WFS1-related disorder
Inborn genetic diseases

Research Path Decision Tree

ΔΔG < 2  + binding site affected   →  CATEGORY 3 — docking experiments
ΔΔG 2–4                            →  CATEGORY 2 — pharmacological chaperones
ΔΔG > 4                            →  CATEGORY 1 — gene therapy
pLDDT < 50                         →  CATEGORY 5 — IDR, experimental only
Stable fold + functional site hit  →  CATEGORY 4 — site-specific docking

Final Schema Categorization

Category 4 — Stable Fold, Function Disrupted

<strong>Category 4 — Stable Fold, Function Disrupted</strong><br/><br/>|ΔΔG|=0.24 negligible. Likely site-specific functional disruption — docking strategy.

Why this card matters. Wolframin's fold survives this substitution (|ΔΔG|=0.24 kcal/mol). The pathogenic signal is real — AlphaMissense places it at 0.344. Protein still folds, but a specific local site is broken. Pharmacological chaperones and small-molecule binders are the rational therapeutic vector.

Files in this folder

AF-O76024-F1-model_v6.pdb — AlphaFold structure
L286P_molstar_viewer.html — interactive 3D viewer (auto-highlights position 286 with ball-and-stick + neighbors within 5Å)
L286P_variant_card.md — this card (source of truth)
L286P_variant_card.html — styled printable card
L286P_dynamut2_summary.html — clean offline DynaMut2 result card
dynamut2_result.json — structured result data
dynamut2_result_page.html — local snapshot of the Biosig result page (asset URLs absolutized)
L286P_wildtype_interactions.pse / L286P_mutant_interactions.pse — PyMOL sessions

Generated by wolfram-variant-card skill · RareResearch.AI Molecular Atlas Every assumption documented. Every score sourced.

Therapeutic Strategy Handoff · prediction

Feed this card to Wolfram Intelligence

Download the L286P PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.

Download L286P PDF card ↓Strategies are AI-generated predictions, not validated therapeutics.