RareResearch.AI
← Back to atlas

S855P

Category 5 — IDR ExclusionConflictingLumenal · predictedσ-1 candidateEditorial
SerineProline at position 855 · C-terminal lumenal domain (653-869) · WFS1 (Wolframin)

Ser→Pro p855 lumenal AM=0.09 ddg=-0.08 pLDDT=45. ClinVar Conflicting evidence. Atlas mechanism: see structural analysis.

Interactive 3D Structure

Wild-type reference
Wild-type S855 — hydrogen bond to T857
Fullscreen ↗
DynaMut2 mutant · S855P
Mutant P855 — polar contact contact to T857 lost
Fullscreen ↗

Bond changes · DynaMut2 interaction analysis

0 lost2 gained2 preserved
Interaction typeWild-type partnerMutant partnerStatus
Hydrogen bondQ853Gained
Hydrogen bondT857T857Preserved
Polar contactQ853Gained
Polar contactT857T857Preserved

Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.

Computational Predictions

DynaMut2 ΔΔG
-0.08kcal/mol
Destabilising — mild
AlphaMissense
0.091
LBen
AlphaFold pLDDT
45
model confidence
Schema
Cat 5
Category 5 — IDR Exclusion
ΔΔG confidence: Reduced confidence · Lumenal
  • pLDDT 45.12 below 70

The underlying AlphaFold model has pLDDT below 70 at this position, so the structure the stability calculation was run on is itself uncertain.

Do not rank this value against a variant from a different region on ΔΔG alone.

Clinical Evidence

ClinVar classificationConflicting classifications of pathogenicity
Review statuscriteria provided, conflicting classifications
Associated conditions(no specific conditions catalogued)
InheritanceConflicting ClinVar classifications.
Population frequency (gnomAD v4)Absent from gnomAD v4
cDNA changec.2563_2565delinsCCG
ClinVar accessionVCV000591341
Last evaluated2020/07/18 00:00

Not observed in ~730k individuals — consistent with a rare allele (ACMG PM2_supporting).

Classified for1★ unverified submission

ClinVar classifies this variant as Conflicting classifications of pathogenicity for Wolfram syndrome (classic) (autosomal recessive, OMIM 222300). Classic Wolfram syndrome is recessive: a single copy does not produce it, and this classification says nothing about a carrier's own risk.

  • Wolfram syndrome (classic)autosomal recessive · OMIM 222300submitted as: Wolfram syndrome 1
  • Submitters conflict on this classification (1★). Treat the conditions above as submitted, not as documented phenotypes.
  • Phenotype scope is interpretation of submitted records. The computed values on this card (AlphaMissense, DynaMut2 ΔΔG, pLDDT) are measurements of the model, not of a patient.
  • Presentation cannot be generalised from neighbouring variants: one nucleotide over can present very differently. Structural similarity supports a mechanistic hypothesis, never a phenotype claim.

Review status: 1★ conflicting classifications. ClinVar "conditions" is the union of all submissions; 0–1★ entries are submitted conditions, not documented phenotypes.

Population frequency
Global (all ancestries)
Absent from gnomAD v4
Homozygotes
Not available

No population breakdown — the variant is absent from gnomAD v4, so no ancestry group has an observed frequency.

No homozygotes — the variant is absent from gnomAD v4 altogether.

Source: gnomAD r4, retrieved 2026-08-21. Values are allele frequencies. The carrier estimate is derived from the allele frequency (Hardy-Weinberg, ~2·AF) and is an approximation, not a published figure — where a published carrier frequency exists it outranks this column and is cited on the card.

Structural Context

Position analysis: LEU854 (2.5 Å), PRO856 (2.5 Å — adjacent existing P! Pro-Pro motif!), THR857 (4.1 Å). Pro-Pro motif created at 855-856. pLDDT 45 IDR boundary. The Atlas's neighbor extraction surfaces this variant's contacts.

Amino-acid chemistry
proline introduction
Position in the protein
C-terminal lumenal domain

Druggability Assessment

Cat 5 IDR boundary — see structural prose. AlphaMissense below threshold (AM under-call class) but mechanism is structurally clear from neighbor analysis. Therapeutic strategy: site-directed at the contacts identified above.

Why this matters

Pro-Pro motif at IDR boundary.
Therapeutic Strategy Handoff · prediction

Feed this card to Wolfram Intelligence

Download the S855P PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.

Download S855P PDF card ↓Strategies are AI-generated predictions, not validated therapeutics.

UniProt Domain Annotation

Chain1890 · Wolframin
Topological domain653869 · Lumenal