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V871M

Category 4 — Stable Fold, Function DisruptedConflictingTransmembrane · predictedEditorial
ValineMethionine at position 871 · TM11 (870-890), helical transmembrane · WFS1 (Wolframin)

Valine → Methionine at position 871 inside TM11. ClinVar Conflicting including WFS1 spectrum + monogenic diabetes. AlphaMissense 0.14 (below threshold) — AM under-call. DynaMut2 ΔΔG -0.15. Same position as V871G.

Interactive 3D Structure

Wild-type reference
Wild-type V871 — hydrogen bond to V875
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DynaMut2 mutant · V871M
Mutant M871 — hydrogen bond to W867 lost (5 contacts lost)
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Bond changes · DynaMut2 interaction analysis

5 lost4 gained6 preserved
Interaction typeWild-type partnerMutant partnerStatus
Hydrogen bondW867W867Preserved
Hydrogen bondR868Gained
Hydrogen bondA874A874Preserved
Hydrogen bondV875V875Preserved
Polar contactW867W867Preserved
Polar contactR868Gained
Polar contactG873Lost
Polar contactA874A874Preserved
Polar contactV875V875Preserved
Van der WaalsI421Gained
Van der WaalsW867Lost
Van der WaalsG873Lost
Van der WaalsA874Lost
HydrophobicI421Gained
HydrophobicV875Lost

Lost / gained / preserved interatomic contacts at the variant residue, from the DynaMut2 (Arpeggio) interaction analysis of the wild-type and energy-minimized mutant structures.

Computational Predictions

DynaMut2 ΔΔG
-0.15kcal/mol
Destabilising — mild
AlphaMissense
0.141
LBen
AlphaFold pLDDT
74
model confidence
Schema
Cat 4
Category 4 — Stable Fold, Function Disrupted
ΔΔG confidence: Reduced confidence · Transmembrane
  • transmembrane position; predictor benchmarked on soluble proteins

This variant sits in the transmembrane band. The stability value was computed with a predictor benchmarked on water-soluble proteins. Published benchmarks of ddG predictors on membrane proteins report correlation below 0.4. Treat the magnitude as unreliable and the sign as weak evidence only.

Do not rank this value against a variant from a different region on ΔΔG alone.

Clinical Evidence

ClinVar classificationConflicting classifications of pathogenicity
Review statuscriteria provided, conflicting classifications
Associated conditionsWFS1-Related Spectrum Disorders; Monogenic diabetes
InheritanceMulti-phenotype.
Population frequency (gnomAD v4)Low frequency · AF 0.993%
cDNA changec.2611G>A
ClinVar accessionVCV000095325
Last evaluated2026/03/01 00:00

Observed in the general population.

Classified for1★ unverified submission

ClinVar classifies this variant as Conflicting classifications of pathogenicity for WFS1-related spectrum (unresolved mode) (dominant or recessive); WFS1-related diabetes (autosomal dominant); Wolfram syndrome (classic) (autosomal recessive, OMIM 222300); Wolfram-like syndrome (autosomal dominant, OMIM 614296); Cataract 41 (autosomal dominant, OMIM 116400); Nonsyndromic hearing loss (DFNA6/14/38) (autosomal dominant, OMIM 600965). ClinVar records this under a WFS1-spectrum label that spans both disease families — recessive Wolfram syndrome (biallelic) and the dominant WFS1-related disorders. The label does not resolve which applies, and it is not a severity statement.

  • WFS1-related spectrum (unresolved mode)dominant or recessivesubmitted as: WFS1-Related Spectrum Disorders
  • WFS1-related diabetesautosomal dominantsubmitted as: Monogenic diabetes; Type 2 diabetes mellitus
  • Wolfram syndrome (classic)autosomal recessive · OMIM 222300submitted as: Wolfram syndrome 1; Wolfram syndrome 1
  • Wolfram-like syndromeautosomal dominant · OMIM 614296submitted as: Wolfram-like syndrome
  • Cataract 41autosomal dominant · OMIM 116400submitted as: Cataract 41
  • Nonsyndromic hearing loss (DFNA6/14/38)autosomal dominant · OMIM 600965submitted as: Autosomal dominant nonsyndromic hearing loss 6; Autosomal dominant nonsyndromic hearing loss 6
  • Submitters conflict on this classification (1★). Treat the conditions above as submitted, not as documented phenotypes.
  • Phenotype scope is interpretation of submitted records. The computed values on this card (AlphaMissense, DynaMut2 ΔΔG, pLDDT) are measurements of the model, not of a patient.
  • Presentation cannot be generalised from neighbouring variants: one nucleotide over can present very differently. Structural similarity supports a mechanistic hypothesis, never a phenotype claim.

Review status: 1★ conflicting classifications. ClinVar "conditions" is the union of all submissions; 0–1★ entries are submitted conditions, not documented phenotypes.

Population frequency
Global (all ancestries)
AF 0.993% · 16,024 / 1,613,120 alleles
Homozygotes
92
Highest-frequency population
Finnish · AF 1.39%

Highest in Finnish: AF 1.39% (873 of 62,980 alleles), in line with the global figure.

92 homozygotes reported in gnomAD v4 (7 Finnish; 79 European (non-Finnish); 2 Ashkenazi Jewish; 2 Remaining individuals; 1 Admixed American; 1 South Asian). gnomAD excludes individuals with severe pediatric disease, so homozygotes in this dataset argue against a fully penetrant severe recessive phenotype and belong in the clinical picture. This is interpretive signal, not a classification.

Ancestry groupAllele freq.AC / ANHom.Carrier est.
Finnish1.39%873 / 62,9807~1 in 36
European (non-Finnish)1.19%14,043 / 1,180,00479~1 in 42
Ashkenazi Jewish0.817%242 / 29,6042~1 in 61
Remaining individuals0.798%499 / 62,5002~1 in 63
Admixed American0.347%208 / 60,0241~1 in 140
African / African American0.187%140 / 75,0660~1 in 270
Amish · under-sampled0.110%1 / 9120
Middle Eastern0.049%3 / 6,0620~1 in 1010
South Asian0.016%15 / 91,0881~1 in 3040

Source: gnomAD r4, retrieved 2026-08-21. Values are allele frequencies. The carrier estimate is derived from the allele frequency (Hardy-Weinberg, ~2·AF) and is an approximation, not a published figure — where a published carrier frequency exists it outranks this column and is cited on the card.

Structural Context

Position 871 same neighbors as V871G: HIS872 (2.5 Å), THR870 (2.5 Å), TRP867 (3.7 Å).

V871M conservative chemistry at the V871 position. AM 0.14 under-call; multi-phenotype confirms.

Amino-acid chemistry
Valine (V) → Methionine (M) — branched aliphatic replaced by flexible sulfur-containing hydrophobic.
Position in the protein
TM11 (residues 870–890) · position 871 (pLDDT 74). Same as V871G.

Druggability Assessment

Category 4 — Stable Fold, Function Disrupted (AM under-call). |ΔΔG| 0.15. AlphaMissense 0.14 below threshold but multi-phenotype confirms.

Mechanism: methionine chemistry shift at TM11 start. Therapeutic: same TM11 cluster.

Why this matters

V871M + V871G at same position — TM11 cluster continues to grow.
Therapeutic Strategy Handoff · prediction

Feed this card to Wolfram Intelligence

Download the V871M PDF below and upload it to Wolfram Intelligence to generate therapeutic-strategy proposals — guanidinium mimetics, sigma-1 agonist docking, NAC thiol-capping. NAC is already on the bench-testing list.

Download V871M PDF card ↓Strategies are AI-generated predictions, not validated therapeutics.

UniProt Domain Annotation

Chain1890 · Wolframin
Transmembrane870890 · Helical
Natural variant871871 · in dbSNP:rs71532874